generation of cisplatin-resistant ovarian cancer cell lines

Authors

nastaran nikounezhad

maryam nakhjavani

adepartment of toxicology and pharmacology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran. farshad hosseini shirazi

pharmaceutical sciences research center, shahid beheshti university of medical sciences, tehran, iran.

abstract

ovarian cancer is the most lethal gynecological cancer in which cisplatin-based treatment plays fundamental role as the first line chemotherapy option. however, development of platinum-resistance is a critical and poorly understood problem in ovarian cancer treatment. although in vitro generation of platinum-resistant ovarian cancer cell lines is a long established approach to uncover the molecular mechanisms underlying resistance development, the methodology of this resistance induction is poorly explained in publications. the aim of this study was to propose a method for induction of resistance in ovarian cancer cell lines. to this purpose, a2780 human ovarian cancer cell line was continuously exposed to stepwise increasing concentrations of cisplatin (0.5–2.6µm) over a period of 6 months and three resistant sublines were collected. cisplatin resistance was examined by clonogenic survival assay and growth curve analysis was carried out in order to evaluate the proliferation characteristics of the established sublines. the a2780 resistant sublines exhibited 5.1 to 11.7 fold resistance to cisplatin, as compared to their parental cells and although growth rate and plateau saturation density significantly decreased by cisplatin resistance enhancement, all three resistant sublines presented a typical growth curve even though they were cultured in the cisplatin containing medium. these results suggest that reliable drug resistant human ovarian cancer cell lines can be successfully established by this method.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Generation of Cisplatin-Resistant Ovarian Cancer Cell Lines

Ovarian cancer is the most lethal gynecological cancer in which cisplatin-based treatment plays fundamental role as the first line chemotherapy option. However, development of platinum-resistance is a critical and poorly understood problem in ovarian cancer treatment. Although in vitro generation of platinum-resistant ovarian cancer cell lines is a long established approach to uncover the molec...

full text

Generation of Cisplatin-Resistant Ovarian Cancer Cell Lines

Ovarian cancer is the most lethal gynecological cancer in which cisplatin-based treatment plays a fundamental role as the first line chemotherapy option. However, development of platinum-resistance is a critical and poorly understood problem in ovarian cancer treatment. Although in vitro generation of platinum-resistant ovarian cancer cell lines is a long established approach to uncover the mol...

full text

Induction of apoptosis in cisplatin-sensitive and -resistant human ovarian cancer cell lines.

In this study, we have assessed the mechanism of cytotoxicity in a series of cisplatin-sensitive and -resistant ovarian carcinoma cells following treatment with equitoxic concentrations of cisplatin. The specific proteolytic degradation and the enzymatic activities of the DNA-dependent protein kinase (DNA-PK) were assessed in the cisplatin-sensitive A2780 cell line and two resistant derivative ...

full text

Biological comparison of ovarian cancer resistant cell lines to cisplatin and Taxol by two different administrations.

Drug resistance is one of the major obstacles to chemotherapy of ovarian cancer. Studies with cell lines can serve as an initial screen for agents that might modulate drug resistance. To establish more appropriate models of drug resistance and explore whether the differences exist in the different drug resistant sublines selected by different treatments, we induced SKOV3 cell line using cisplat...

full text

Increased gene-specific repair of cisplatin interstrand cross-links in cisplatin-resistant human ovarian cancer cell lines.

We have studied several aspects of DNA damage formation and repair in human ovarian cancer cell lines which have become resistant to cisplatin through continued exposure to the anticancer drug. The resistant cell lines A2780/cp70 and 2008/c13*5.25 were compared with their respective parental cell lines, A2780 and 2008. Cells in culture were treated with cisplatin, and the two main DNA lesions f...

full text

Cisplatin inhibits paclitaxel-induced apoptosis in cisplatin-resistant ovarian cancer cell lines: possible explanation for failure of combination therapy.

Combination chemotherapy using paclitaxel with a platinum-based regimen is currently the standard first-line therapy for ovarian cancer after surgical cytoreduction. Whereas cisplatin-paclitaxel combination chemotherapy has shown significant efficacy over previous drug combinations in ovarian cancer, 20-30% of patients fail to respond to this combination. These patients are deemed cisplatin-pac...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of pharmaceutical sciences

جلد ۱۲، شماره ۱، صفحات ۱۱-۲۰

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023